IgE Tests | IgE Blood Allergy Testing | Human Allergen IgE Blood Tests

Immediate allergenic reactions in patients are commonly associated with elevated circulating plasma levels of IgE antibodies specific to their corresponding allergens. Common allergens include pollens, molds, yeasts, fungal spores, dust mites, other airborne inhalants, common foods, insect stings and bites, and in rare cases certain drugs and cosmetics. ALerCHEK’s Allergen Specific Human IgE test incorporates the use of microwell plastic strips, which have been coated with allergen proteins. Serum or plasma is exposed to the microwell and the bound IgE antibody is detected using an enzyme (HRP) labeled anti-human IgE antibody. Our own extremely sensitive TMB/Peroxide Substrate (Colorburst™ Blue) is used to detect the levels of enzyme present, which is directly proportional to the level of specific IgE bound to the specific allergen. This general method has been classically termed the RAST method.

We offer over 400 stock allergens, however, we have the capacity to generate or aquire and coat additional allergens upon request. We normally use a twelve well strip format with each well coated with a different allergen. Eight of these strips are organized into a standard microwell frame holder. In other words, eight patients may be tested for 12 allergens per 96 well strip plate. We have the flexibility of offering many types of variations on this same theme based on customer requests. This type of flexibility is required due to the seasonality and variability of allergen prevalence in various parts of the world.